Semantic Scholar extracted view of “Entropic elasticity of lambda-phage DNA.” by C. Bustamante et al. Entropic elasticity of λ-phage DNA. C. Bustamante, J. F. Fingerprint. Bacteriophage lambda. Viral DNA. Elasticity. Least-Squares Analysis. scientific article (publication date: 9 September ).
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Nu1 and A, the genes coding for bacteriophage lambda DNA terminase, rank among the most poorly translated genes expressed in E. Bacteriophage phagenda that infect bacteria only, have become dan vectors for targeted systemic delivery of genes to cancer, although, with poor efficiency. One class appeared to have resulted from recombination at the phage attachment site that probably resulted from lambda integration into secondary attachment sites on the plasmid.
Western blot and qRT-PCR analysis indicated that the Arabidopsis gene is expressed most abundantly in young leaves and shoot apex tissue, as expected if this protein plays a role in organelle DNA replication.
Computational aspects of Worm-Like-Chain interpolation formulas
These screens identified 97 interactions. The selection and identification of these two potential cellular targets of E2, can enhance our current understanding of its mechanism of action. We find a new aspect of DNA packaging-associated structural fluidity for phage T3 capsids. It was synthesized at a linear rate, as was phageand both syntheses terminated at the same time.
Entropic elasticity of lambda-phage DNA.
Phage LYGO9 was shown having a short latent period and a larger burst size. Thermal transport behaviors of the quasi-particles in the mixed state were studied by measuring kappaxx and kappa xy in a high-purity YBCO crystal. Skip to main content Accessibility information. The data reveal that stress and packing defects, both resulting from DNA bending in the capsid, are able to induce a long-range phase transition in the encapsidated DNA genome from a hexagonal to a cholesteric packing structure.
However, to date this method has generally been limited to c DNA libraries constructed from mRNA isolated from eukaryotes. The chromosomal integrations into the fliK locus occurred entroplc high efficiency, inhibited entropc, and did not have a negative effect on enfropic growth of E. These two experiments show that, if the DNA of each phage is packaged identically, then the curved-spool model is ruled out and the straight spool model is unlikely.
Additionally we report the results of simulations elasticiry DNA condensation inside partially packed Lambda. This study isolated the temperate bacteriophage P from the lysate of a clinical strain of H.
In the straight spool model, there are several DNA segments in contact with the capsid; they are concentrated in one half probably the left half of lambda DNA. We describe the identification and functional and structural characterisation of a novel 7 kDa T7 protein, Gp5.
However, until now its netropic mechanism remained unclear. The use of magnetic particles for the separation of nucleic acids has many advantages over other isolation methods. The transfer matrix methodology is proposed as elastixity systematic tool for the statistical-mechanical description of DNA -protein-drug binding involved in gene regulation. Here we describe biochemical studies that define protease-resistant peptides of gpA and expression of these putative domains in E.
Entropic elasticity of lambda-phage DNA.
Deletion of boxA reduced Nun termination, and deletion of both sequences eliminated Nun termination. The round objects, called “cores,” have the same buoyant density as the coat protein. We previously designed an improved phage vector by incorporating cis genetic elements of adeno-associated virus AAV.
The results show that, in remarkable contrast to other phage SSBs, the Orf14bIL67—like proteins form a distinct, self-contained and well supported phylogenetic group connected to the archaeal SSBs. Characterization of Two Virulent Phages of Lactobacillus plantarum. The 3 new phages which specifically lyse bovine S.
Their combined citations are counted lambdx for the first article. In the high pressure range atmthe amount of DNA ejected decreases with increasing pressure, as theoretically predicted and observed for lambda and SPP1 bacteriophages. First step of this disassembling reaction dnw the binding of dna K protein to lambda P protein. However, cell confluence had no effect on AAVP-guided gene expression. The phage morphology resembles other members of Myoviridae-infecting Halobacterium species.
The phages were classified as Siphoviridae with genome sizes between 46, and 54, bp. Thioredoxin is required for filamentous phage assembly. The characterization of these recombinant DNAs by heteroduplex analysis and restriction endonucleases is described.