Adhatoda vasica belonging to Acanthaceae family, commonly known as Adosa, Adhatoda vasica Flowers, Antibacterial activity, Antifungal activity, Diffusion. Abstract. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Antibacterial activity of leaf extract of Adhatoda vasica was studied against Bacillus subtilis, Antibacterial activity was determined by disc diffusion method.

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A human adenocarcinoma cancer cell line was obtained from National Institute of Cell Sciences, Pune. UV-Visible spectra were taken in methanol on Thermo Fisher instrument.

Classical Uses of Medicinal Plants. Journal of Natural Product and Adhaatoda Resources. Potent uterine activity of alkaloid vasicine. Vasicine acetate showed moderate antibacterial activity compared to Vasicine.

The following Gram negative, Gram positive bacteria, clinical isolates, and fungi were used for the experiment: Introduction Natural products still remain the most important source for discovery of new and potential drug molecules. The combined chloroform extract was washed with water, dipped, and dried over anhydrous sodium sulphate. BHT is used as standard of antioxidant. Streptomycin was used antibacteral positive control for bacteria and Ketoconazole as positive control for fungi.

Open in a separate window. B revised New Delhi, India: Vasicine acetate showed minimum inhibitory concentration values against bacteria: International Journal of Cancer. The MIC for fungi was defined as the lowest extract concentration, showing no visible fungal growth after incubation time.


Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

The extract was filtered through Whatman No. Acivity activity of two natural alkaloids, vasicine acetate and 2-acetyl benzylamine, isolated from Indian shrub Adhatoda vasica Ness. Published online Jan Evaluation of antioxidant activity of isoferulic acid in vitro.

Publications and Information Directorate; Structure of the compounds: The crude extract of A.

Shade dried and coarsely powdered leaves of A. The emergence of drug-resistant pathogens and the increase in diseases affecting the immune system have greatly intensified the need to investigate new bioactive metabolites for potential pharmaceutical and industrial applications [ 12 ]. Nature acts as a prominent reservoir for new and novel therapeutics.

Cytotoxic A and antimicrobial effects of Methylobacterium sp. It was studied for antimicrobial, antioxidant, and anticancer activities.

Antibacterial activity of adhatoda vasica leaf extract

Materials and Methods 2. Antibacterial and antifungal activities were carried out using disc diffusion method [ 16 ]. Table 2 Minimum inhibitory concentration of Vasicine acetate against tested antibafterial and fungi. Balachandran2 S. Effect of Adhatoda vasica massage in pyorrhoea.

After various durations of cultivation, the solution in the medium was removed. The required concentrations, Vasicine acetate showed antimycobacterial activity [ 15 ]. Vasicine acetate exhibited good zone of inhibition against bacteria: The physical and vascia data were compared with those reported in the literature [ 2122 ].


In vitro antibacterial activity of selected medicinal plants from lower Himalayas.

Two mobile phases A and B were used at flow rate of 1. Results and Discussion The ethanol extract of A.

The antifungal agent Ketoconazole for fungi and antibacterial agent Streptomycin for bacteria were included in the assays as positive controls. The plant was identified by Dr. Estimates of worldwide burden of cancer in Essential oils of the leaves of A.

Extraction Shade dried and coarsely vawica leaves of A.

In vitro antibacterial activity of selected medicinal plants from lower Himalayas.

Antimicrobial Activity Antibacterial and antifungal activities were carried out using disc diffusion method [ 16 ]. Indian Journal of Medical Research. The alkaloid Vasicine was isolated from ethanolic extract of the leaves of A.

The free radical scavenging activity was calculated as follows:. The radical scavenging activity of Vasicine acetate at different concentrations is shown in Table 3. Separation was carried out at room temperature. Other constituents include vitamin C, saponins, flavonoids as well as steroids, and fatty acids [ 13 ].

The worldwide threat of antimicrobial resistance.